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Ayed Alshammari, Sampson Agyapong Atuahene, Qian Xu Sheng, Muhammad Jamil, Sami Ullah, Shakir Ullah, Muhammad Kashif, Muhammad Qasim, Obaid Ullah, Mourad Ben Said
Spatio-temporal epidemiological survey reveals high infestation and extensive species diversity of hard ticks infesting camels from Pakistan
Camel production is severely hampered in Pakistan by a wide range of diseases, including ticks and tick-borne pathogens. Camels serve as hosts for various tick-borne pathogens that can lead to human illnesses. Thereby, it was necessary to identify tick species and their infestation prevalence on camels before recommendation of any tick control measures. A total of 1800 engorged and semi-engorged ticks were collected from 1000 camels. In total, tick infestation intensity and abundance were 3.191 |
2023 |
https://www.sasas.co.za/journals/spatio-temporal-epidemiological-survey-reveals-high-infestation-and-extensive-species-diversity-of-hard-ticks-infesting-camels-from-pakistan/ |
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Mr Jawad Ahmad, Mr Arab Hussain, Mr Amjad Ullah, Mr Ahmad Yar, Mr Subhan Ullah, Mr Zeshan Ali, Dr Huang Yun-ha, Dr Hamid sattar, Dr Tariq Hussain, Dr Hamayun khan, Dr Ihsan Ali, Ali Gohar, Dr Obaid Ullah, Prof Nan-zhu Fang, Dr Yanya Fang, Prof Zhongshu Li
Hematological Profile of Achai Cows during different Stages of Pregnancy in Kohistan Hindukush Mountainous Range.
Pregnancy is a common situation and concurrently the most altered physiologic state consisting of variety
of physiological and hormonal changes. These changes begin after conception which alters the normal
physiological status of blood. The present study was conducted to evaluate changes in hematological
biomarkers of Achi cows at different stages of pregnancy. Forty multiparous cows with 4 ± 1 years of
age and live weight of 188.23 ± 2.6 to 207.13 ± 2.59 kg of different stages of pregnancy were |
2023 |
https://dx.doi.org/10.17582/journal.pjz/20210629060605 |
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Noor Bad shah Khattak, Hamayun Khan, Muhammad Tarique Tunio, Shakoor Ahmad, Nazir Ahmad, Syed Murtaza Hassan Andrabi, Obaid Ullah, Ihsan Ali, Nan-zhu Fang.
Dose-dependent expression of green tea (Camellia Sinensis) extracts in the bovine semen extender for post-thawed fertility indicators.
This study describes antioxidative activity of green tea (GT) extract on standard post-thawed quality
indicators of frozen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three
weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of
GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied
were the percentage of motility, survivability, plasma membrane integrity and acrosome integrit |
2022 |
https://dx.doi.org/10.17582/journal.pjz/20181230051216. |
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Farhad Ali, Ijaz Ahmad, Muhammad Ijaz Ali, Muhammad Hasnain Riaz, Nadar Khan, Obaid Ullah, Faizul Hassan, Syed Muhammad Suhail, Rajwali Khan, Muhammad Tahir Khan, Irshad Ahmad & Muhammad Tariq Zeb.
Mitochondrial phylogenetic and diversity analysis in Azi-Kheli buffalo
The present study was conducted for the first time in Pakistan to investigate Cytochrome C Oxidase
Subunit 1 (CO1) gene and full-length Displacement Loop (D-loop) region of mitochondrial DNA in Azi-Kheli buffalo breed
native to northern hilly areas of Khyber Pakhtunkhwa Province of Pakistan. The present study was designed to investigate
phylogeny and diversity in Azi-Kheli buffalo, through two mitochondrial DNA regions, i.e., Cytochrome C Oxidase Subunit-I
(CO1) and Displacement Loop (D-loop) re |
2021 |
https://doi.org/10.1007/s11250-021-02949-z. |
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Obaid ULLAH, Zhongshu LI, Ihsan ALI1, Lijie XU1, Haixing LIU1, Syed Zahid Ali SHAH and Nanzhu FANG
Pterostilbene alleviates hydrogen peroxide-induced oxidative stress via nuclear factor erythroid 2 like 2 pathway in mouse preimplantation embryos.
Pterostilbene (PTS) in blueberries is a phytoalexin with antioxidant properties. PTS exerts strong cytoprotective
effects on various cells via Nuclear Factor Erythroid 2 like 2 (NFE2L2) pathway. We evaluated the antioxidant PTS treatment in
mouse preimplantation embryos. In vitro culture media were supplemented with different concentrations of PTS. Treatment of
zygotes with 0.25 μM PTS improved the development of day 4 blastocysts (P < 0.05). Moreover, H2O2 treatment significantly
increased the |
2019 |
10.1262/jrd.2018-089 |
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Xu Lijie, Obaid Ullah, Liu Haixing, Ihsan Ali, Zhongshu Li and Nan-Zhu Fang.
Pioglitazone Mediated Reduction in Oxidative Stress and Alteration in Level of PPARγ, NRF2 and antioxidant Enzyme Genes in Mouse Preimplantation Embryo during Maternal to Zygotic Transition.
Excessive accumulation of reactive oxygen species (ROS) is one of the reasons for the slow growth
of mammalian preimplantation embryos. Peroxisome proliferators-activated receptor gamma (PPARγ)
functions in the nuclear regulatory factor 2 (Nrf2) antioxidant pathways by binding to the promoters
of antioxidant genes and regulates the expression of genes that associate with NADPH oxidase. To
understand the role of PPARγ in the development of early embryos and define the mechanism responsible
for th |
2019 |
http://dx.doi.org/10.17582/journal.pjz/2019.51.3.1085.1092 |
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Obaid Ullah. Zhongshu Li. Ihsan Ali. Lijie Xu. Haixing Liu. Hua-Zhi Jin. Yan-Ya Fang. Qing-Guo Jin. Nanzhu Fang
Pterostilbene exerts a protective effect via regulating tunicamycin-induced endoplasmic reticulum stress in mouse preimplantation embryos
Pterostilbene (PTS) mainly enriched in small fruits such as berries and grapes exerts an antioxidant effect. However, the
protective effects of PTS against endoplasmic reticulum stress (ERS) have not yet been elucidated in mouse preimplantation
embryo. ERS plays an important role in regulating the pathological and physiological processes, including embryonic development.
We explored the protective effect of PTS on the tunicamycin (TM)-induced ERS in mouse preimplantation embryos.
In vitro, cultu |
2019 |
10.1007/s11626-018-0308-9. |
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Ihsan ALI, Hai Xing LIU, Li ZHONG-SHU, Ma DONG-XUE, Lijie XU, Syed Zahid Ali SHAH, Obaid ULLAH and Fang NAN-ZHU. Reduced glutathione alleviates tunicamycin induced endoplasmic reticulum stress in mouse preimplantation embryos
Reduced glutathione alleviates tunicamycin induced endoplasmic reticulum stress in mouse preimplantation embryos
Endoplasmic reticulum (ER) stress, a dysfunction in protein-folding capacity, is involved in many pathological
and physiological responses, including embryonic development. This study aims to determine the developmental competence,
apoptosis, and stress-induced gene expression in mouse preimplantation embryos grown in an in vitro culture medium
supplemented with different concentrations of the ER stress inducer tunicamycin (TM) and the antioxidant glutathione (GSH).
Treatment of zygotes with 0.5 |
2018 |
10.1262/jrd.2017-055 |
|
Obaid Ullah, Li Zhongshu, Ihsan Ali, Lijie Xu, Haixing Liu, Nanzhu Fang
Effects of pterostilbene on the activation of nuclear factor erythroid 2-related factor 2 pathway during in vitro maturation of mouse oocytes
Pterostilbene (PTS) is a natural polyphonic compound known to have biological activities, such as antioxidant
and anticancer effects. This study was designed to regulate the effect of pterostilbene on the in vitro maturation
(IVM) of mouse oocytes denuded of the cumulus (DOs). Different concentration of PTS was added to IVM
media with immature DOs. After maturation, meiosis II (MII) stage rates oocytes, Measurement of reactive
oxygen species (ROS) and glutathione (GSH) levels, activation of the |
2018 |
https://doi.org/10.5539/jas.v10n7p35 |
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Ihsan ALI, Syed Zahid Ali SHAH, Jin Yi, Obaid ullah, Fang NAN-ZHU.
Reactive oxygen species-mediated unfolded protein response pathways in preimplantation embryos.
Excessive production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress-mediated responses are critical to embryonic
development in the challenging in vitro environment. ROS production increases during early embryonic development with the increase in
protein requirements for cell survival and growth. The ER is a multifunctional cellular organelle responsible for protein folding, modification,
and cellular homeostasis. ER stress is activated by a variety of factors including R |
2017 |
https://doi.org/10.4142/jvs.2017.18.1.1 |
